of the instant invention can be used in place of standard RNA or DNA oligomers. For example, the target-specific polymers of the invention can be used in a variety o f diagnostic assays for detection of RNA or DNA having a given target sequence. Further, the polymers of the invention also have potential use as therapeutic agents.
5380645 GENERALIZED METHOD FOR ASSESSMENT OF COLORECTAL CARCINOMA Vogelstein Bert Baltimore, MD, UNITED STATES Assigned to The Johns Hopkins University A method is provided for assessing the generalized genetic change which occurs during tumorigenesis. The method relies on measurement of loss of a large number of alleles from the chromosomes of the tumor cells. The alleles are RFLP markers. The role of any of the particular alleles in tumorigenesis need not be known. The amount of ailelic loss allows a prognosis to be made regarding tumor metastasis, tumor recurrence, and mortality.
5380653 EXPRESSION VECTORS AND METHODS FOR INTRACELLULAR PROTEIN PRODUCTION IN BASCILLUS Palva llkka Helsinki, FINLAND Assigned to The Finnish National Public Health Institute The invention relates to recombinant DNA molecules and to methods for producing proteins by means of said molecules. Particularly, the present invention relates to recombinant DNA molecules which are capable of being synthesized in Bacillus strain bacteria comprising the regulation and deleted non-functional signal sequence of the a-amylase gene of B. amyloliquefaciens, or a substantial part thereof, to which sequence a structural gene of any desired homologous or heterologous protein or peptide may be joined. These recombinant DNA molecules can be used, for example, to achieve intracelhilar expression of any desired protein or peptide in Bacillus s~ain bacteria.
5380655 METHODS AND COMPOSITIONS FOR THE PRODUCTION OF HAEMOPHILUS INFLUENZAE TYPE B MAJOR OUTER MEMBRANE PROTEIN ANTIGENS Hansen Eric J Piano, TX, UNITED STATES Assigned to Board of Regents The University of Texas S),stem Disclosed are DNA and other biological compositions, including biological cultures, for preparing Haemophihis influenzae antigens by recombinant means. The disclosed recombinant antigens are suitable for use in the preparation of immunogenic compositions for use in vaccination against various Haemophilus infhienzae type B infections. Particular embodiments disclosed include DNA segments which encode the P2 major outer membrane protein antigen, also referred to as the 39/38 protein, biological cultures transformed by these DNA segments, and the preparation of recombinant P2 antigen through the use of these biological cultures.
5380657 METHOD FOR ISOLATION OF INSERTION ELEMENTS FROM CORYNEFORM BACTERIA Schaefer Andrea; Seep-Feldhaus Anna-Hildegard; Jaeger Wolfgang; Kalinowski Joeru; Wohlleben Wolfgang; Puehler Alfred Bielefeld, GERMANY Assigned to Degussa Aktiengesellsehaft A method locating insertion elements (IS elements) or transposons in coryneform bacteria, a positive selection system suitable for the above, the IS elements found in this manner and their use, is disclosed. The method involves: (1) The construction of a non-self-transferrable vector mobilizable from an E. coil mobilizer strain which vector is composed of (a) A DNA segment containing a replicon functional in E. coil, (b) A second DNA segment containing the DNA fragment coding for the mobilization function (Mob site containing the oriT), (c) A third DNA segment which recombines bomologously in Gram-positive bacteria and/or contains a replicon functional in coryneform bacteria, (d) A D N A segment from Bacillus subtiliscontaining the sacB
PATENT ABSTRACTS gens, (2) Transfer of Otis vector by means of conjngative transfer into the coryneform recipient straius, (3) Cultivation of the transconjugants containing the vector in an -10% sucrosecontaining nutrient medium, (4) Lysis of the sucrose-resistaat clones, cleaving of the plasmids with restriction endonncleases and analysis of the fragments.
Biologically active proviral molecular clones of bovine immunodeficiency-like virus and cell lines infected with the same have been prepared. Various utilities of the clones are described.
5380831 SYNTHETIC INSECTICIDAL CRYSTAL PROTEIN GENE
TRUNCATED HUMAN SERUM ALBUMIN POLYPEPTIDES AS PLASMA EXPANDING AGENTS
Adang Michael J; Rocheleau Thomas A; Merlo Donald; Murray Elizabeth E Madison, WI, UNITED STATES Assigned to Mycogen Plant Science Inc
Ballance David J; Hinchliffe Edward; Geisow Michael J; Senior Peter Attenborcugh, UNITED KINGDOM Assigned to Delta Biotechnology Limited
Synthetic Bacillus thuringieusis toxin genes designed to be expressed in plants at a level higher than naturally-occusring Bt genes are provided. These genes utilize codons preferred in highly expressed monocot or dicot proteins.
Polypeptides corresponding to mature human serum albumin residues 1 to n,where n is between 369 and 419 inclusive, are useful as substitutes for albumin in the treatment of burns and shock in humans, the clearance of undesirable compounds, (such as bilirubin) from human blood, in laboratory growth media and in HSA assays. The polypeptides may be produced by recombinant DNA techniques, especially in yeast.
~80~3 POLYNUCLEOTIDE REAGENTS CONTAINING SELECTABLE CLEAVAGE SITES Urdea Michael S Alamo, CA, UNITED STATES Assigned to Chiron Cot'txzation
5380727 SYNERGISTIC COMBINATION FOR TREATING HERPES INFECTIONS Deziel Robert; Guindon Yvan Ville Mont Royal, CANADA Assigned to Bio-Mega Inc Disclosed herein is a combination of an antiviral nucleoside analog and a ribonucleotide reductase inhibiting peptide derivative. The combination is useful for treating herpes infections.
5380830 MOLECULAR CLONES OF BOVINE IMMUNODEFICIENCYLIKE VIRUS Gonda Matthew A Walkcrsville, MD, UNITED STATES Assigned to The United States of America as represented by the Secretary of the Department of Health and Human Services
Novel methods for assaying a nucleic acid analyte are provided, which employ polynucleotides having oligonucleotide sequences substantially homologous to a sequence of interest in the analyte, where the presence or absence of hybridization at a predetermined stringency provides for the release of a label from a support. Particularly, various techniques are employed for binding a label to a support, whereupon cleavage of either a single or double strand, a label may be released from a support,where the release of the label can be detected as indicative of the presence of a particular oligonudeutide sequence in a sample. The method finds use in diagnosis of disease, genetic monitoring, and analysis of nucleic acid mixtures.
5380836 NUCLEIC ACID ENCODING SODIUM CHANNEL PROTEIN Rogart Richard Chicago, IL, UNITED STATES Assigned to Arch Development Corporation