SUBCUTANEOUS GLUCOSE ELECTRODE
DATA ANALYSIS METHOD FOR USE WITH FLUORESCENT BACTERIAL SENSORS
Heller Adam; Pishko Michael V UNITED STATES
A small diameter flexible electrode designed for subcutaneous in vivo amperometric monitoring of glucose is described. The electrode is designed to allow one-point in vivo calibration, i.e., to have zero output current at zero glucose concentration, even in the presence of other electroreactive species of serum or blood. The electrode is preferably three or four-layered, with the layers serially deposited within a recess upon the tip of a polyamide insulated gold wire. A first glucose concentration-to-current transducing layer is overcoated with an electrically insulating and glucose flux limiting layer (second layer) on which, optionally, an immobilized interference-eliminating horseradish peroxidase based film is deposited (third layer). An outer (fourth) layer is biocompatible.
5593853 GENERATION AND SCREENING OF SYNTHETIC DRUG LIBRARIES Chen Hao; Radmer Richard Adelphia, MD, UNITED STATES Assigned to Martek Corporation Methods are provided for generating highly diverse mixtures of compounds which may be screened for biological activities. Once the activity is fotmd, the component of the mixture which is responsible for the activity can be isolated by fractionation and assay for the biological activity. Polyhydroxylated organic monomers and oligomers are used as starting materials for generating the fibraries.
Bemdt Klans Stewartstown, PA, UNITED STATES Assigned to Becton Dickinson and Company A method of analyzing the data provided by a fluorescent chemical sensor calculates a ratio based on the AC and DC components of the emission from the chemical sensor. This ratio, or the emission modulation, will change shouM bacterial growth be ongoing in the vial. The calculated ratio is compared to a desired value. The desired value is selected to focus the operation of the system into a Mgh resolution area for the sensor. If the calculated and desired ratios differ, then the frequency of the excitation radiation is changed until the calculated and desired ratios are effectively equal. At that time the adjusted frequency is measured. By focusing the desired ratio into a high resolution area, and adjusting the frequency until the system reaches that ratio one ensures that all readings are performed at a high resolution area of the sensor. The adjusted frequency is utifized to provide an indication of whether the particular vial is experiencing bacterial growth. This adjusted frequency is a linear function of changing conditions within the vial. Several methods of achieving the above features of this invention are disclosed. By utilizing this method, one provides an easily interpreted indication of whether a particular fluorescent chemical sensor is in a vial which is experiencing bacterial growth.
5593867 FLUORERSCENCE POLARIZATION DETECTION OF NUCLEIC ACID AMPLICATION Walker G Terrance; Nadeau James G; Linn C Preston Chapel Hill, NC, UNITED STATES Assigned to Becton Dickinson and Company Fluorescence polarization methods for detection of nucleic acid amplification. A fluoreacently labelled ofigodeoxynucleotide probe is converted