Association of apolipoprotein E gene polymorphism with serum lipid levels

Association of apolipoprotein E gene polymorphism with serum lipid levels

SYMPOSIUM 9: BIOMARKERS AND DIAGNOSTIC TOOLS O9-7 Association of apolipoprotein E gene polymorphism with serum lipid levels Sehrish Fatima ∗ , Syed S...

59KB Sizes 0 Downloads 122 Views

SYMPOSIUM 9: BIOMARKERS AND DIAGNOSTIC TOOLS

O9-7 Association of apolipoprotein E gene polymorphism with serum lipid levels Sehrish Fatima ∗ , Syed Shahid, Obaid Khan, Abid Azhar University of Karachi, Pakistan

Dyslipidemia has been marked to play an integral role in the development of cardiovascular diseases. Various environmental and genetic factors may influence dyslipidemia. Polymorphism of the apolipoprotein E (apo E) gene may influence lipid metabolism by modulating lipid levels. This study was designed to investigate the role of genetic variants of apo E in dyslipidemic patients. The study was carried out in equal number (n = 110) of normal subjects and dyslipidemic patients. Genotyping was performed by Polymerase Chain Reaction and Restriction Fragment Length Polymorphism (PCR- RFLP) at Single Nucleotide Polymorphisms (SNPs)

S38

www.elsevier.com/locate/nbt

New Biotechnology · Volume 31S · July 2014

rs429358 (ApoE4) and rs7412 (ApoE2). The genotype frequencies were: E3/E3 (75%), E2/E3 (4.1%), E3/E4 (20%), E4/E4 (0%), E2/E4 (0%), and E2/E2 (0.45%). The allele frequencies were 0.025, 0.875 and 0.1 for ␧2, ␧3 and ␧4, respectively. In control group allele frequencies for ␧2, ␧3 and ␧4 were 0.045, 0.83 and 0.127, respectively, whereas in dyslipidemic patients group allele frequencies for ␧2, ␧3 and ␧4 were 0.045, 0.682 and 0.273 respectively. Total cholesterol (TC) and low density lipoprotein cholesterol (LDL-C) levels were significantly high in ␧4 allele carriers (p < 0.001) and high density lipoprotein cholesterol (HDL-C) level was high in ␧2 allele carriers (p < 0.05) indicating protective effect of ␧2 allele. Apo E polymorphism has significant influences on lipid profile in dyslipidemic patients group as frequency of ␧4 allele was found to be prevalent in patients group which indicates a relationship of ␧4 allele in apo E gene with dyslipidemia. http://dx.doi.org/10.1016/j.nbt.2014.05.1698