Introduction to mass spectrometry

Introduction to mass spectrometry

ANALYTICAL BIOCHEMISTRY155,2 12-2 l4 ( 1986) BOOK REVIEWS cursory treatment which is not well written. There is a major section on modes of ionizatio...

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ANALYTICAL BIOCHEMISTRY155,2 12-2 l4 ( 1986)

BOOK REVIEWS cursory treatment which is not well written. There is a major section on modes of ionization and strategyfor data interpretation which is useful to the novice trying to interpret the significance of his recorded data. The earlier edition of this book, published in 1976, was The reader is taken through several illustrative spectra, directed to the biological chemist and was limited in its and the advantages and disadvantages of various ionization content. This new edition has an additional 100 pages of techniques are presented. information which have been integrated with the former Although this book can be recommended for its commaterial with considerable reorganization of the text, pro- pleteness and could be a useful text for introductory courses viding a new and different book. For example, the section in mass spectrometry, there are some shortcomings. The on desorption ionization covers approximately 20 pages graphic representations of spectra and chromatograms are and is entirely new material accompanied by almost 400 of uneven quality and frequently poorly located in the references. Literature documentation throughout the text text. The reader has to make comparisons between figures is excellent, with complete author and title citations given located on different pages and, in one case, printed in opin each chapter. posing directions. The book begins with an introductory chapter to acSome of the old graphics from the first edition, especially quaint the reader with common terms, and then proceeds the alternating voltage presentations, should have been through two chapters of qualitative and quantitative ex- redrawn. There are a considerable number of typographical amples of how mass spectrometry may be applied to solve errors, for which the publisher must share some responvarious types of problems. These chapters are an excellent sibility. way for the novice to peruse a number of applications and More troubling than the lack of editorial review of the be guided into the literature on any particular subject. figures is the confusing and awkward style of the text. As Some of the examples are new, but most are repeated from a reviewer familiar with the subject, I sometimes found the earlier edition. the prose style baffling. For example, the time-of-flight There are four chapters on instrumentation covering section was explained clearly to me by a colleague in several the available mass spectrometers, sample inlet systems, sentences, but Watson’s text remains puzzling. Other pordetectors, and means of data acquisition. The material is tions of the text, which were retained from the first edition, generally up-to-date, and while not all forms of instruwere not smoothly incorporated into this edition with rementation are reviewed with the same thoroughness, the sulting redundancies. The prose style suggests this book wealth of literature citations assiststhe reader desiring mom was written in bits and pieces and finally assembled on a complete coverage. The section on tandem instrumentacomputer. The consequence of the choppy style and awktion appears skimpy to this reviewer (3 pages), especially ward prose is that this text cannot be recommended for in contrast to the time-of-flight section (7 pages). Sample its intended readership, but may accompany a formal inlet systemsare succinctly presented; in the case of thercourse as a useful reference text. mospray, an example of an application would have been SANFORD P. MARKEY a valuable addition. The chapter on data acquisition is a Introduction to Mass Spectrometry, 2nd ed. By J. [email protected] WATSON, Raven Press, New York, 1985. 375 pp. $44.50.

of Immunofluorescence in Cultured Cells. By MARK C. WILLINGHAM AND IRA PASTAN, Academic Press, Orlando, Fla., 1985. 186 pp. $36.50.

An Atlas

In this small volume Dr. Willingham and Dr. Pastan have assembled a substantial collection of representative immunofluorescent images produced by antibodies to cell proteins in cultured cells. The first section of the book is devoted to immunofluorescence techniques. Included are the mechanics of processing, fixation, pcrmeabilization, reagents, controls, and photography all in a form that is very brief and readable. At the end of this section is a protocol for routine immunofluorescence of cultured cells 0003-2697/86 $3.00 Copyright0 I986 by Academic All rights of reproduction

F’res, Inc. in any form rem’wd.


and some useful references. After some representative bright field images in Section 2, the authors present a large section (3) devoted to immunofluorescent images of antibodies localized to the various cell structures: plasma membrane, cytoplasmic, organelles, nucleus, and nucleolus. Most of the images are of antigens in fibroblastic and epithelioid cell lines. Lists of antibody sources, antigens, and references are included in addition to a very useful index. The volume is highly recommended to those biochemists or cell biologists seeking a practical introduction to immunofluorescent localization in cultured cells. JOHN A. HANOVER