Molecular cell genetics

Molecular cell genetics

ANALYTICAL BIoOlEMlSTRY 154, 37 1 (1986) BOOK Molecular Cell GOTTESMAN. Genetics. Edited Wiley-Interscience, by MICHAEL New York, REVIEWS...

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ANALYTICAL

BIoOlEMlSTRY

154, 37 1 (1986)

BOOK Molecular

Cell

GOTTESMAN.

Genetics.

Edited

Wiley-Interscience,

by

MICHAEL

New

York,

REVIEWS with geneiic manipulations of CHO cells. This includes valuable information concerning maintenance ofthese cells in culture, cell fusion, and cell sorting. Additionally, emerging methods for the introduction of foreign genes into CHO cells by vector mediated gene transfer are outlined. The third section presents genetic systems already developed in CHO lines including some for intermediary metabolism. cell structure and behavior. and mechanisms of genetic variation. This section is very detailed and provides a generous overview of the scope of somatic cell genetics in a number of biological systems. The appendices alone may provide justification for this volume. In Appendix I the lineages of CHO cell line are provided. Appendix II lists C’HO mutants according to phenotype with appropriate references. All in all, this book is carefully prepared and should be a valuable resource for those interested in aspects of somatic cell genetics.

M.

1985.

631 pp. This volume represents an important resource for the practicing cell biologist and somatic cell geneticist. A wide variety of genetic systems developed in Chinese hamster cell lines are discussed with an emphasis on the details of mutant selection and characterization. Some 37 authors have contributed to the text. The first section of the book presents the historical developments in the establishment of Chinese hamster cells used for somatic cell genetlcs. After an initial chapter describing the biology and genetics of the Chinese hamster itself. the establishment of the Chinese hamster ovary cell (CHO). Chinese hamster lung cell (V79). and the Chinese hamster embryo libroblast line (CHEF) cell lines are described. This is fiJIlowed by a discussion of the genetic map of the Chinsse hamster. The second section deals

High

Performance

Liquid

Chromatography

istry. Edited by A. HENSCHEN. PEICH, AND W. VOELTER. VCH

Beach.

Fla..

K. HUPE. Publishers.

JOHN

A. HANOVER

the material is a little out ofdate. In the chapter on biogenic amines. for example. most of the 210 references were from 1970-1975. with the most recent reference being from 19X I It was also of interest to note that the structure of the product obtained with o-phthaladehyde and amines was not consistent throughout the book (pp. 155 and 339) and neither of the two references to this important derivatization reagent was listed in the index. The strong points of the book are the width and depth of the coverage of the analysis of a very wide range of biochemicals in complex matrices that can be rapidly translated into a working method in the reader‘s own laboratoq. The major weak points of the volume are that the literature references are too old considering the many advances that have been made in the last IO year\ and that alternate methods of analysis are not critically compared.

in BiochemF. LOTTSDeerfield

1985. 638 pp. $88.00.

This morh consists of 14 chapters of which the lirst three ( 138 pages) are devoted to the theory of chromatography. types of columns. and the mechanics of instrumentation. The remainder of the hook is fairly evenly divided into chapters on amino acids and proteins. peptide hormones. hiogenic amines. lipids. carbohydrates. nucleotides, porphyrins. steroid hormones. vitamins. organic acids in humans. and secondary plant constituents. Most of the chapters are subdivided by the type of column used in the separation (e.g.. reversed-phase. ion-exchange) and each of these sections is further subdivided into groups determined by the chemical structure of the test compound. The coverage of the material in each chapter is very comprehensive and well documented. but

JOHN

371

0003~2697/86

K. BAKER

$3.00

Copynghf (0 1986 by Academic Press. Inc. Ail rights of reproduction in any form reserved.