E631 JACC March 27, 2012 Volume 59, Issue 13
Arrhythmias VENTRICULAR ARRHYTHMIAS FOLLOWING INTRAMYOCARDIAL INJECTIONS OF BONE MARROW STEM CELLS: AN ANIMAL MODEL ACC Moderated Poster Contributions McCormick Place South, Hall A Monday, March 26, 2012, 9:30 a.m.-10:30 a.m.
Session Title: Ventricular Arrhythmias in 2012: From the J-Wave and Early Repolarization to LVADs Abstract Category: 17. Arrhythmias: VT Presentation Number: 1241-361 Authors: Anna Vittoria Mattioli, Roberto Lonardi, Sonia Pennella, Enrico Giuliani, Alberto Farinetti, University of Modena and Reggio Emilia, Modena, Italy, Istituto Nazionale di Ricerche Cardiovascolari, Bologna, Italy Background: The concern that intramyocardial(i.m.)delivery of cells could cause potentially life-threatening ventricular arrhythmias has been raised. Aim of the study was to evaluate the pro-arrhythmic effects of Bone Marrow Stem Cells (BMSCs) injections in a pre-clinical model. Methods: In New Zealand rabbits an ischemia/reperfusion damage was induced by temporary ligation of anterior descending coronary artery. Homologous BMSCs were isolated,cultured and re-suspended for injection. We compared i.m. injections of BMSCs with intravenous(i.v.) administration. A control group was treated with i.m. injections of saline, to evaluate pro-arrhythmic effect of puncture. The hourly number of ventricular premature contractions(VPC), QT interval time and QTc time were recorded and calculated. Results: After cell injection, temporary but frequent VPC were observed in the i.m. BMSCs group as compared with i.m. saline and i.v. injections of cells. QTc time interval was prolonged during ischemia and recovered in control and in the group treated with i.v. BMSCs whereas it remained longer in rabbits treated with i.m. BMSCs. (see table) Conclusions: BMSCs injections were associated with changes in cardiac electrophysiological properties as shown by QTc prolongation. The combination of cell clusters behaving as barriers to electrical impulse propagation and electrical remodeling contribute to the development of arrhythmias. Results (*p<0.01) VPC baseline VPC 7 days QTc interval pre-ischemia QTc interval during-ischemia QTc interval post-ischemia
BMSCs i.m. injections 11 +/- 4 132 +/- 65 422 +/- 16 432 +/- 21* 429 +/- 19*
BMSCs i.v. injections 13 +/- 6 54 +/- 20 419 +/- 20 427 +/- 23* 421 +/- 19
Saline i.m. injections 14 +/- 5 34 +/- 7 420 +/- 25 434 +/- 28* 426 +/- 29*